Composite

Part:BBa_K3089013:Design

Designed by: Aiai Dong   Group: iGEM19_Greatbay_SCIE   (2019-10-14)


csgA-linker-mfp5-mfp5-His fusion protein for yeast expression


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 274
    Illegal PstI site found at 23
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 274
    Illegal PstI site found at 23
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 274
    Illegal XhoI site found at 244
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 274
    Illegal PstI site found at 23
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 274
    Illegal PstI site found at 23
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Redesign the sequence to make it suitable for yeast expression


Source

mfp5 - Mytilus galloprovincialis yeast - Pichia pastoris

References